ige  (Chondrex Inc)

Journal: Science Advances

Article Title: A beneficial environment promotes immune resilience through epigenetic regulation

doi: 10.1126/sciadv.ady7317

Figure Lengend Snippet: ( A ) Airway resistance measured after increasing concentrations of nebulized methacholine to anesthetized mice. ( B ) Representative BAL differential counts and PAS staining images and ( C ) quantification of BAL eosinophilia and ( D ) volume of mucus within airway epithelium. ( E ) Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis from whole-lung homogenates detecting the expression of Il13 gene. ( F ) Serum titers of anti-OVA–specific IgE. ( G ) Annotated immune cells were subsetted from whole-lung scRNA-seq and 2D plotted using uniform manifold approximation and projection for dimension reduction (UMAP). ( H ) OVA and OVA + FD immune cell UMAP representation overlaid. ( I ) DEG number comparing OVA versus OVA + FD groups. ( J ) Representative flow cytometry plots highlighting MDM and DC identification and ( K and L ) quantification of frequency of these cell types within parent gates. Results are plotted as means ± SEM, and difference between means were compared using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test ( n = 6 to 9).

Article Snippet: Mouse CCL8 (BioLegend, no. 446904), human CCL8 (BioLegend, no. 442204), OVA-specific IgE (Chondrex, no. 3004), and OVA-specific IgG1 (Chondrex, no. 3013) were used according to the supplier’s protocols.

Techniques: Staining, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Flow Cytometry

Journal: Science Advances

Article Title: A beneficial environment promotes immune resilience through epigenetic regulation

doi: 10.1126/sciadv.ady7317

Figure Lengend Snippet: ( A ) Volcano plot showing DEGs in OVA versus OVA + FD MDMs, analyzed by model-based analysis of single-cell transcriptomics (MAST). ( B ) Ccl8 gene expression in OVA versus OVA + FD. ( C ) Mouse serum CCL8 measured by enzyme-linked immunosorbent assay. ( D ) Serum CCL8 levels in human patients with asthma versus healthy controls. ( E ) WT mice were sensitized to OVA and treated daily with anti-CCL8 antibody or isotype 6 hours before OVA challenge. Representative BAL Giemsa cytospins and PAS-stained lung sections are shown. ( F ) Quantification of BAL eosinophils and ( G ) volume of mucus. ( H ) Lung inflammatory eosinophils (CD45 + , Ly6g neg , CD125 int , SiglecF +, CD11c + , and CD101 + ) quantified by flow cytometry. ( I and J ) CCL8 gene expression in murine BMDMs and human monocytes after 48 hours of FD exposure followed by 6 hours of LPS stimulation. ( K ) Average gene expression from MHCII-related genes ( H2-Eb1 , H2-Ab1 , H2-Aa , and Ciita ) were down-regulated in OVA + FD MDMs. ( L ) MHCII mean fluorescence intensity (MFI) on lung MDMs (CD45 + , F4/80 + , Ly6G neg , CD11b + , and CCR2 + ). ( M ) HLA-DR MFI in human monocyte–derived APCs exposed to FD for 48 hours and LPS for 6 hours. ( N ) BMDMs pulsed with OVA 323–339 peptide after FD and LPS stimulation and then cocultured with naive CD4 + T cells. T cell proliferation assessed by CellTrace™ Violet (CTV) MFI-based division index. ( O ) Proliferation-associated gene ( Cenpe , Top2a , and Mki67 ) average expression within CD4 + T cell subset in OVA versus OVA + FD. Modules were clustered by KEGG/Reactome terms; antigen presentation node shows altered interaction strength. Results are plotted as means ± SEM, and difference between means were compared using one-way ANOVA followed by Tukey’s post hoc test ( n = 4 to 19).

Article Snippet: Mouse CCL8 (BioLegend, no. 446904), human CCL8 (BioLegend, no. 442204), OVA-specific IgE (Chondrex, no. 3004), and OVA-specific IgG1 (Chondrex, no. 3013) were used according to the supplier’s protocols.

Techniques: Single-cell Transcriptomics, Gene Expression, Enzyme-linked Immunosorbent Assay, Staining, Flow Cytometry, Fluorescence, Derivative Assay, Expressing, Immunopeptidomics